Reconfirmation of Galba cubensis (Gastropoda: Lymnaeidae), host of Fasciola hepatica (Trematoda: Digenea), by molecular techniques in the Dominican Republic
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Jan 16, 2020
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Abstract
Among the freshwater gastropods, many of the species of the family Lymnaeidae are involved in the transmission of the parasite trematode Fasciola hepatica (Linnaeus, 1758) worldwide. Galba cubensis (Pfeiffer, 1839) has been reported as a vector in South America and other areas of the Neotropics. The use of molecular techniques has been used by different groups of researchers to discriminate between species with conquiological similarity, such as those of the Galba/Fossaria group, clarifying epidemiological aspects of crucial importance in the study and control of fasciolosis. The objective of this research was to reconfirm with molecular techniques the presence of G. cubensis and distinguish it from other cryptic species such as Galba truncatula (Müller, 1774) and Galba shirazensis (Küster, 1862) in the northeastern and southern region of the Dominican Republic. Mollusks phenotypically compatible with G. cubensis were captured and conquiological measurements were taken. In addition, conventional PCR DNA analysis was carried out with specific primers for the three suspected species, with specimens fixed in 80 % alcohol. Amplification was obtained from a mitochondrial DNA region of the species G. cubensis only. The use of the technique confirmed that G. cubensis is, to date, the only lime species involved in the transmission of fasciolosis in the Dominican Republic.
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freshwater mollusks, limneid, intermediate host, fasciolosis, Greater Antilles
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Alba, A., A. A. Vázquez, H. Hernández, J. Sánchez, R. Marcet, M. Figueredo, J. Sarracent y J. Fraga. 2015. A multiplex PCR for the detection of Fasciola hepatica in the intermediate snail host Galba cubensis. Veterinary Parasitology, 211 (3-4): 195?200.
Alda, P., M. Lounnas, A. A. Vázquez, R. Ayaqui, M. Calvopiña, M. Celi-Erazo, R. T. Dillon, Jr., P. Jarne, E. S. Loker, F. C. Muñiz Pareja, J. Muzzio-Aroca, A. O. Nárvaez, O. Noya, L. M. Robles, R. Rodríguez-Hidalgo, N. Uribe, P. David, J. P. Pointier y S. Hurtrez-Boussès. 2018. A new multiplex PCR assay to distinguish among three cryptic Galba species, intermediate hosts of Fasciola hepatica. Veterinary Parasitology, 251: 101?105.
Álvarez-Vicioso, J. M. y E. T. Cordero de Castellanos. 1977. Faciolasis bovina en la República Dominicana. Ed. Universidad Autónoma de Santo Domingo, Santo Domingo: República Dominicana, 189 pp.
Armúa-Fernández M. T., O. Castro, O. Correa, L. Carvalho, A. Mangold, J. Sanchís y J. M. Venzal. 2016. First molecular characterization of Galba neotropica in Uruguay. Revista FAVE – Sección Ciencias Veterinarias, 15: 9?13
Bargues, M. D., P. Artigas, Y. Mera, R. L. Sierra, J. P. Pointier y S. Mas-Coma. 2007. Characterisation of Lymnaea cubensis, L. viatrix and L. neotropica n. sp., the main vectors Fasciola hepatica in Latin America, by analysis of their ribosomal and mitochondrial DNA. Annals of Tropical Medicine and Parasitology, 101 (7): 621?641.
Correa, A. C., J. S. Escobar, O. Noya, L. E. Velásquez, C. González-Ramírez, S. Hurtrez-Boussès y J. P. Pointier. 2011. Caracterización morfológica y molecular de Lymnaeidae (Gastropoda: Hygrophila) de la región neotropical. Biomédica, 31(sup.3): 201?206.
De Vargas Castro, M., J. Martínez La Hoz, J. Castillo, D. Paulino, P. M. Alarcón-Elbal y A. A. Vázquez. 2019. Potencialidad de Pseudosuccinea columella (Say, 1817) (Molusca: Gastropoda: Lymnaeidae) en la transmisión de la fascioliasis humana en República Dominicana. Ciencia y Salud, 3 (2): 9?16.
Doyle, J. J. y J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11–15.
Gómez, J. D, M. Vargas y E. A. Malek. 1986. Freshwater molluks of the Dominican Republic. The Nautillus, 100 (4): 130?134.
Hawley, D. M. y S. M. Altizer. 2011. Disease ecology meets ecological immunology: understanding the links between organismal immunity and infection dynamics in natural populations. Functional Ecology, 25: 48?60.
Lounnas, M., A. A. Vázquez, P. Alda, K. Sartori, J. P. Pointier, P. David y S. Hurtrez-Boussès, 2017. Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae). Journal of Molluscan Studies, 83 (1): 63?68.
Malek, E. A. 1985. Snail Hosts of Schistosomiasis and other Snail-Transmitted Diseases in Tropical America: a Manual. Pan American Health Organization, Washington: USA, 325 pp.
Martínez, M. A., P. Artigas, L. Mateo, V. Chagas, S. Adam, S. Mas-Coma y M. D. Bargues. 2015. Identificación de Lymnaea cubensis en zonas endémicas de Fasciolasis en República Dominicana. XIX Congreso de la Sociedad Española de Parasitología: 23, 24 y 25 de julio de 2015; Vitoria-Gasteiz (España). SOCEPA; 2015.
Mas-Coma, S. 2005. Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology, 79 (3): 207?216.
Mas-Coma, M. S., J. G. Esteban y M. D. Bargues. 1999a. Epidemiologi?a de la fascioliasis humana: revisio?n y propuesta de nueva clasificacio?n. Boleti?n de la Organizacio?n Mundial de la Salud, Recopilacio?n de arti?culos N°1: 70?76.
Mas-Coma, S., R. Anglés, J. G. Esteban, M. D. Bargues, P. Buchon, M. Franken y W. Strauss. 1999b. The Northern Bolivian Altiplano: A región highly endemic for human fasciolasis. Tropical Medicine and International Health, 4 (6): 454?467.
Mas-Coma, S., I. R. Funatsu y M. D. Bargues. 2001. Fasciola hepatica and lymnaeid snails occurring at very high altitude in South America. Parasitology, 123 Suppl: S115?27.
Mas-Coma, S., M. A. Valero y M. D. Bargues. 2008. Effects of climate change on animal and zoonotic helminthiases. Revue Scientifique et Technique (International Office of Epizootics), 27: 443?457.
Mas-Coma, S., M. A. Valero y M. D. Bargues. 2009a. Climate change effects on trematodiases, with emphasis on zoonotic fascioliasis and schistosomiasis. Veterinary Parasitology, 163 (4): 246?280.
Mas-Coma, S., M. A. Valero y M. D. Bargues. 2009b. Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Advances in Parasitology, 69: 41?146.
Narváez, A. O., J. Muzzio Aroca, P. Alda, V. Macías Castro, M. Lounnas, S. Hurtrez-Boussès, O. Noya, L. Martini Robles y J. P. Pointier. 2017. Primer reporte de Galba cubensis (Gastropoda: Lymnaeidae) en el Ecuador, hospedador potencial de Fasciola hepatica en arrozales de la costa ecuatoriana. El Misionero del Agro, 13 (4): 36?47.
Paraense, W. L.1983. Lymnaea columella in northern Brazil. Memórias do Instituto Oswaldo Cruz, 78 (4): 477?482.
Pointier, J. P., N. J. Cazzaniga, C. González-Salas, A. Gutiérrez, J. A. Arenas, M. D. Bargues y S. Mas-Coma. 2006. Anatomical studies of sibling species within neotropical lymnaeids, snail intermediate hosts of fascioliasis. Memórias do Instituto Oswaldo Cruz, 101 (4): 431?435.
Pointier, J. P., O. Noya, B. Alarcón de Noya y A. Théron, 2009. Distribution of Lymnaeidae (Mollusca: Pulmonata) intermediate hosts of Fasciola hepatica in Venezuela. Memórias do Instituto Oswaldo Cruz, 104 (5): 790?796.
Schniebs, K., P. Gloer, S. Quiñonero-Salgado, J. López-Soriano y A. K. Hundsdoerfer. 2018. The first record of Galba cubensis (L. Pfeiffer, 1839) (Mollusca: Gastropoda: Lymnaeidae) from open fields of Europe. Folia Malacologica, 26 (1): 3?15.
Standley, C. J., L. Prepelitchi, S. M. Pietrokovsky, L. Issia, J. R. Stothard y C. Wisnivesky-Colli. 2013. Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina. Parasites & Vectors, 6 (1): 304.
Vázquez, A.A, J. Sánchez, J. P. Pointier, A. Théron y S. Hurtrez-Boussès. 2014. Fasciola hepática in Cuba: compatibility of different isolates with two intermediate snail hosts, Galba cubensis and Pseudosuccinea columella. Journal of Helminthology, 88 (4): 434?440.
Vázquez, A. A., M. Lounnas, J. Sánchez, A. Alba, A. Milesi y S. Hurtrez-Boussès. 2016. Genetic and infective diversity of the liver fluke Fasciola hepatica (Trematoda: Digenea) from Cuba. Journal of Helminthology, 90 (6): 719?725.
Vázquez, A. A, P. Alda, M. Lounnas, E. Sabournin, J. P Pointier y S. Hurtrez-Boussés. 2018. Lymnaeidae snails host of Fasciola hepatica and Fasciola gigantica (Trematoda: Digenea): A wordlwide review. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 13 (062): 1?15.
Alda, P., M. Lounnas, A. A. Vázquez, R. Ayaqui, M. Calvopiña, M. Celi-Erazo, R. T. Dillon, Jr., P. Jarne, E. S. Loker, F. C. Muñiz Pareja, J. Muzzio-Aroca, A. O. Nárvaez, O. Noya, L. M. Robles, R. Rodríguez-Hidalgo, N. Uribe, P. David, J. P. Pointier y S. Hurtrez-Boussès. 2018. A new multiplex PCR assay to distinguish among three cryptic Galba species, intermediate hosts of Fasciola hepatica. Veterinary Parasitology, 251: 101?105.
Álvarez-Vicioso, J. M. y E. T. Cordero de Castellanos. 1977. Faciolasis bovina en la República Dominicana. Ed. Universidad Autónoma de Santo Domingo, Santo Domingo: República Dominicana, 189 pp.
Armúa-Fernández M. T., O. Castro, O. Correa, L. Carvalho, A. Mangold, J. Sanchís y J. M. Venzal. 2016. First molecular characterization of Galba neotropica in Uruguay. Revista FAVE – Sección Ciencias Veterinarias, 15: 9?13
Bargues, M. D., P. Artigas, Y. Mera, R. L. Sierra, J. P. Pointier y S. Mas-Coma. 2007. Characterisation of Lymnaea cubensis, L. viatrix and L. neotropica n. sp., the main vectors Fasciola hepatica in Latin America, by analysis of their ribosomal and mitochondrial DNA. Annals of Tropical Medicine and Parasitology, 101 (7): 621?641.
Correa, A. C., J. S. Escobar, O. Noya, L. E. Velásquez, C. González-Ramírez, S. Hurtrez-Boussès y J. P. Pointier. 2011. Caracterización morfológica y molecular de Lymnaeidae (Gastropoda: Hygrophila) de la región neotropical. Biomédica, 31(sup.3): 201?206.
De Vargas Castro, M., J. Martínez La Hoz, J. Castillo, D. Paulino, P. M. Alarcón-Elbal y A. A. Vázquez. 2019. Potencialidad de Pseudosuccinea columella (Say, 1817) (Molusca: Gastropoda: Lymnaeidae) en la transmisión de la fascioliasis humana en República Dominicana. Ciencia y Salud, 3 (2): 9?16.
Doyle, J. J. y J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11–15.
Gómez, J. D, M. Vargas y E. A. Malek. 1986. Freshwater molluks of the Dominican Republic. The Nautillus, 100 (4): 130?134.
Hawley, D. M. y S. M. Altizer. 2011. Disease ecology meets ecological immunology: understanding the links between organismal immunity and infection dynamics in natural populations. Functional Ecology, 25: 48?60.
Lounnas, M., A. A. Vázquez, P. Alda, K. Sartori, J. P. Pointier, P. David y S. Hurtrez-Boussès, 2017. Isolation, characterization and population-genetic analysis of microsatellite loci in the freshwater snail Galba cubensis (Lymnaeidae). Journal of Molluscan Studies, 83 (1): 63?68.
Malek, E. A. 1985. Snail Hosts of Schistosomiasis and other Snail-Transmitted Diseases in Tropical America: a Manual. Pan American Health Organization, Washington: USA, 325 pp.
Martínez, M. A., P. Artigas, L. Mateo, V. Chagas, S. Adam, S. Mas-Coma y M. D. Bargues. 2015. Identificación de Lymnaea cubensis en zonas endémicas de Fasciolasis en República Dominicana. XIX Congreso de la Sociedad Española de Parasitología: 23, 24 y 25 de julio de 2015; Vitoria-Gasteiz (España). SOCEPA; 2015.
Mas-Coma, S. 2005. Epidemiology of fascioliasis in human endemic areas. Journal of Helminthology, 79 (3): 207?216.
Mas-Coma, M. S., J. G. Esteban y M. D. Bargues. 1999a. Epidemiologi?a de la fascioliasis humana: revisio?n y propuesta de nueva clasificacio?n. Boleti?n de la Organizacio?n Mundial de la Salud, Recopilacio?n de arti?culos N°1: 70?76.
Mas-Coma, S., R. Anglés, J. G. Esteban, M. D. Bargues, P. Buchon, M. Franken y W. Strauss. 1999b. The Northern Bolivian Altiplano: A región highly endemic for human fasciolasis. Tropical Medicine and International Health, 4 (6): 454?467.
Mas-Coma, S., I. R. Funatsu y M. D. Bargues. 2001. Fasciola hepatica and lymnaeid snails occurring at very high altitude in South America. Parasitology, 123 Suppl: S115?27.
Mas-Coma, S., M. A. Valero y M. D. Bargues. 2008. Effects of climate change on animal and zoonotic helminthiases. Revue Scientifique et Technique (International Office of Epizootics), 27: 443?457.
Mas-Coma, S., M. A. Valero y M. D. Bargues. 2009a. Climate change effects on trematodiases, with emphasis on zoonotic fascioliasis and schistosomiasis. Veterinary Parasitology, 163 (4): 246?280.
Mas-Coma, S., M. A. Valero y M. D. Bargues. 2009b. Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Advances in Parasitology, 69: 41?146.
Narváez, A. O., J. Muzzio Aroca, P. Alda, V. Macías Castro, M. Lounnas, S. Hurtrez-Boussès, O. Noya, L. Martini Robles y J. P. Pointier. 2017. Primer reporte de Galba cubensis (Gastropoda: Lymnaeidae) en el Ecuador, hospedador potencial de Fasciola hepatica en arrozales de la costa ecuatoriana. El Misionero del Agro, 13 (4): 36?47.
Paraense, W. L.1983. Lymnaea columella in northern Brazil. Memórias do Instituto Oswaldo Cruz, 78 (4): 477?482.
Pointier, J. P., N. J. Cazzaniga, C. González-Salas, A. Gutiérrez, J. A. Arenas, M. D. Bargues y S. Mas-Coma. 2006. Anatomical studies of sibling species within neotropical lymnaeids, snail intermediate hosts of fascioliasis. Memórias do Instituto Oswaldo Cruz, 101 (4): 431?435.
Pointier, J. P., O. Noya, B. Alarcón de Noya y A. Théron, 2009. Distribution of Lymnaeidae (Mollusca: Pulmonata) intermediate hosts of Fasciola hepatica in Venezuela. Memórias do Instituto Oswaldo Cruz, 104 (5): 790?796.
Schniebs, K., P. Gloer, S. Quiñonero-Salgado, J. López-Soriano y A. K. Hundsdoerfer. 2018. The first record of Galba cubensis (L. Pfeiffer, 1839) (Mollusca: Gastropoda: Lymnaeidae) from open fields of Europe. Folia Malacologica, 26 (1): 3?15.
Standley, C. J., L. Prepelitchi, S. M. Pietrokovsky, L. Issia, J. R. Stothard y C. Wisnivesky-Colli. 2013. Molecular characterization of cryptic and sympatric lymnaeid species from the Galba/Fossaria group in Mendoza Province, Northern Patagonia, Argentina. Parasites & Vectors, 6 (1): 304.
Vázquez, A.A, J. Sánchez, J. P. Pointier, A. Théron y S. Hurtrez-Boussès. 2014. Fasciola hepática in Cuba: compatibility of different isolates with two intermediate snail hosts, Galba cubensis and Pseudosuccinea columella. Journal of Helminthology, 88 (4): 434?440.
Vázquez, A. A., M. Lounnas, J. Sánchez, A. Alba, A. Milesi y S. Hurtrez-Boussès. 2016. Genetic and infective diversity of the liver fluke Fasciola hepatica (Trematoda: Digenea) from Cuba. Journal of Helminthology, 90 (6): 719?725.
Vázquez, A. A, P. Alda, M. Lounnas, E. Sabournin, J. P Pointier y S. Hurtrez-Boussés. 2018. Lymnaeidae snails host of Fasciola hepatica and Fasciola gigantica (Trematoda: Digenea): A wordlwide review. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 13 (062): 1?15.
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How to Cite
De Vargas Castro, M., Camejo Jiménez, J. T., Martínez La Hoz, J., Castillo, J. C., Paulino, D., & Alarcón-Elbal, P. M. (2020). Reconfirmation of Galba cubensis (Gastropoda: Lymnaeidae), host of Fasciola hepatica (Trematoda: Digenea), by molecular techniques in the Dominican Republic. Novitates Caribaea, (15), 51–62. https://doi.org/10.33800/nc.vi15.215
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